Science for Education Today, 2021, vol. 11, no. 5, pp. 142–156
© Novikova I. I., Ogudov A. S., Shevkun I. G., Serenko E. V., Chuenko N. F., Gavrish S. M., Semenikhina M. V., Kryuchkova N. Y., 2021
UDC: 
613.26+613.03+616.08+378

Anti-inflammatory, hypoglycemic and hypolipidemic effects of Stevia rebaudiana bertoni leaf extract in an experimental model of diabetes mellitus and obesity in laboratory animals

Novikova I. I. 1 (Novosibirsk, Russian Federation), Ogudov A. S. 1 (Novosibirsk, Russian Federation), Shevkun I. G. 2 (Moscow, Russian Federation), Serenko E. V. 1 (Novosibirsk, Russian Federation), Chuenko N. F. 1 (Novosibirsk, Russian Federation), Gavrish S. M. 1 (Novosibirsk, Russian Federation), Semenikhina M. V. 1 (Novosibirsk, Russian Federation), Kryuchkova N. Y. 3 (Omsk, Russian Federation)
1 Federal Budgetary Institution of Science "Novosibirsk Research Institute of Hygiene" of the Federal Service for Supervision of Consumer Rights Protecti
2 Department of Sanitary Supervision of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
3 Budgetary Institution of Continuing Professional Education of the Omsk region «Center for the Advanced Training of Healthcare Workers»
Full text (Open Access) — Downloaded 818
Abstract: 

Introduction. The paper presents the results of experimental studies focused on assessing the anti-inflammatory, hypoglycemic and hypolipidemic effects of Stevia rebaudiana Bertoni leaf extract. The study was conducted on an experimental model of "zinc" diabetes in white male Wistar rats and under conditions of acute hyperlipidemia caused by the Tween-80 detergent.
The aim of the study was to evaluate the feasibility of using Stevia rebaudiana Bertoni leaf extracts in the diet as a natural sweetener for the organizing nutrition of overweight and diabetic people, including educational settings for children, on the model of induced diabetes mellitus and acute hyperlipidemia in laboratory animals.
Materials and Methods. In order to solve the research problem, the authors utilized the following techniques: assessing the biochemical parameters of blood serum, determining the level of glucose in the blood, lipid formula and delayed-type hypersensitivity reaction. Theoretical methods involved reviewing and analyzing Russian and international academic articles.
Results. The findings of this study suggest that it is possible to use Stevia rebaudiana Bertoni leaf extracts as a natural sugar substitute in the diet of people with overweight and diabetes mellitus, as well as for prophylaxis in healthy people, including meals for children in educational settings.
Conclusions. The article concludes that an immunomodulatory effect and the absence of side effects of the Stevia rebaudiana Bertoni leaf extract confirm its usage in the daily diet of the population.

Keywords: 

Stevia rebaudiana Bertoni; Stevia; Twin-80; Dithizon; Diabetes mellitus; Healthy eating; Food hygiene; Hyperlipidemia; Obesity; Delayed-type hypersensitivity.

URL WoS/RSCI: https://www.webofscience.com/wos/rsci/full-record/RSCI:47136063

For citation:
Novikova I. I., Ogudov A. S., Shevkun I. G., Serenko E. V., Chuenko N. F., Gavrish S. M., Semenikhina M. V., Kryuchkova N. Y. Anti-inflammatory, hypoglycemic and hypolipidemic effects of Stevia rebaudiana bertoni leaf extract in an experimental model of diabetes mellitus and obesity in laboratory animals. Science for Education Today, 2021, vol. 11, no. 5, pp. 142–156. DOI: http://dx.doi.org/10.15293/2658-6762.2105.08
References: 
  1. Dedov I. I., Shestakova M. V., Suntsov Yu. I., Peterkova V. A., Galstyan G. R., Mayorov A. Yu., Kuraeva T. L., Sukhareva O. Yu. Federal targeted programme "prevention and management of socially significant diseases (2007-2012)": results of the "diabetes mellitus" sub-programme. Diabetes mellitus, 2013, no. 2, pp. 2–48. URL: https://elibrary.ru/item.asp?id=18763323
  2. Chattopadhyay S., Raychaudhuri U., Chakraborty R. Artificial sweeteners – a review. Journal of Food Science and Technology, 2014, vol. 51 (4), pp. 611–621. DOI: https://doi.org/10.1007/s13197-011-0571-1
  3. Goyal S. K., Samsher G. R. K., Goyal R. K. Stevia (Stevia rebaudiana) a bio-sweetener: A review. International Journal of Food Sciences and Nutrition, 2010, vol. 61 (1). pp. 1–10. DOI: https://doi.org/10.3109/09637480903193049
  4. Lemus-Mondaca R., Vega-Gálvez A., Zura-Bravo L., Ah-Hen K. Stevia rebaudiana Bertoni, source of a high-potency natural sweetener. A comprehensive review on the biochemical, nutritional and functional aspects. Food Chemistry, 2012, vol. 132 (3), pp. 1121–1132. DOI: https://doi.org/10.1016/j.foodchem.2011.11.140
  5. Jeppesen P. B., Gregersen S., Poulsen C. R., Hermansen K. Stevioside acts directly on pancreatic beta cells to secrete insulin: Actions independent of cyclic adenosine monophosphate and adenosine triphosphate-sensitive K+-channel activity. Metabolism, 2000, vol. 49 (2), pp. 208–214. DOI: https://doi.org/10.1016/S0026-0495(00)91325-8
  6. Jeppesen P. B., Gregersen S., Rolfsen S. E., Jepsen M., Colombo M., Agger A., Xiao J., Kruhøffer M., Ørntoft T., Hermansen K. Antihyperglycemic and blood pressure-reducing effects of stevioside in the diabetic Goto-Kakizaki rat. Metabolism, 2003, vol. 52 (3), pp. 372–378. DOI: https://doi.org/10.1053/meta.2003.50058
  7. Gregersen S., Thomsen J. L., Brock B., Hermansen K. Endothelin-1 stimulates insulin secretion by direct action on the islets of Langerhans in mice. Diabetologia, 1996, vol. 39 (9), pp. 1030–1035. DOI: https://doi.org/10.1007/BF00400650
  8. Philippaert K., Pironet A., Mesuere M., Sones W., Vermeiren L., Kerselaers S., Pinto S., Segal A., Antoine N., Gysemans C., Laureys J., Lemaire K., Gilon P., Cuypers E., Tytgat J., Mathieu C., Schuit F., Rorsman P., Talavera K., Voets T., Vennekens R. Steviol glycosides enhance pancreatic beta-cell function and taste sensation by potentiation of TRPM5 channel activity. Nature Communications, 2017, vol. 8 (1), pp. 14733. DOI: https://doi.org/10.1038/ncomms14733
  9. Choi E. M., Hwang J. K. Effects of Morus alba leaf extract on the production of nitric oxide, prostaglandin E2 and cytokines in RAW 264.7 macrophages. Fitoterapia, 2005, vol. 76, pp. 608–613. DOI: https://doi.org/10.1016/j.fitote.2005.05.006

10. Rulis A. M., Levitt J. A. FDA’S food ingredient approval process. Regulatory Toxicology and Pharmacology, 2009, vol. 53 (1), pp. 20–31. DOI: https://doi.org/10.1016/j.yrtph.2008.10.003

11. Chen J., Bendix P., Reziwanggu J., Stig A., Dyrskog E. U., Colombo M., Hermansen K. Stevioside does not cause increased basal insulin secretion or β-cell desensitization as does the sulphonylurea, glibenclamide: Studies in vitro. Life Sciences, 2006, vol. 78 (15), pp. 1748–1753. DOI: https://doi.org/10.1016/j.lfs.2005.08.012

12. Soejarto D. D., Kinghorn A. D., Farnsworth N. R. Potential sweetening agents of plant origin. III. Organoleptic evaluation of Stevia leaf herbarium samples for sweetness. Journal of Natural Products, 1982, vol. 45 (5), pp. 590–599. DOI: https://doi.org/10.1021/np50023a013

13. Rybakova A. V., Makarova M. N., Kukharenko A. E., Vichare A. S., Ruffer F. R. Current requirements for and approaches to dosing in animal studies. Bulletin of the Scientific Center for Expertise of Medicinal Products, 2018, vol. 8 (4), pp. 207–217. (In Russian) URL: https://www.elibrary.ru/item.asp?id=36565540

14. Airapetova K. A., Sergeeva E. O., Kompantseva E. V. Terekhov A. Yu.,Sadzhaya L. A. Studying of hypolipidemic action of bear s onion extract (ramson) (allium ursinum l.) Bulletin of the Samara Scientific Center of the Russian Academy of Sciences, 2011, vol. 13 (1-4), pp. 758–760. (In Russian) URL: https://www.elibrary.ru/item.asp?id=17305184

15. Mozheyko L. A. Experimental models for studying diabetes mellitus part ii. Surgically, streptozotocin and dithizone-induced diabetes. Journal of Grodno State Medical University, 2013, no. 4, pp. 5–10. (In Russian) URL: https://www.elibrary.ru/item.asp?id=21078313

16. Sorokina N. S., Mikhnevich N. V. Influence of tactivin and myelopid on immune system during development of morphine dependence. International Journal of Applied and Basic Research, 2016, no. 5, pp. 759–760. (In Russian) URL: https://www.elibrary.ru/item.asp?id=25949054

Date of the publication 31.10.2021