Novosibirsk State Pedagogical University Bulletin, 2013, vol. 3, no. 5, pp. 136–143
UDC: 
УДК 57+57.04

The influence of different conditions of illumination on lifespan of Drosophila melanogaster laboratory strains with mutations in genes superoxide dismutase

Shostal O. A. 1 (Syktyvkar, Russian Federation), Moskalev A. 1 (Syktyvkar, Russian Federation)
1 Institute of Biology of the Komi Scientific Centre of the Ural Division of RAS
Abstract: 

Light is a crucial environmental factor influencing living organisms during their whole lives. The genetic mechanisms of light influence on Drosophila melanogaster lifespan are poorly understood. Article is devoted to research of influence of different conditions of illumination (24 h, 12 h, 0 h) on lifespan of Drosophila strains with defect of the genes of the cytoplasmic (Sod1) and mitochondrial (Sod2) superoxide dismutase. It is demonstrated, that individuals of Drosophila with a low ability detoxify free radicals are characterized by a more significant difference between lifespan in the darkness and in the light in compared with individuals of wild type strain. The increase of the photoperiod (from 12 to 24 hours) leads to reduction of lifespan of individuals with mutations in the investigated genes. The given result indicating that the risk of excessive illumination. It was shown the role of superoxide dismutase genes in regulating lifespan of the Drosophila melanogaster fruit fly in response to change of photoperiod.

Keywords: 

lifespan, Drosophila melanogaster, light conditions, superoxide dismutase

For citation:
Shostal O. A., Moskalev A. The influence of different conditions of illumination on lifespan of Drosophila melanogaster laboratory strains with mutations in genes superoxide dismutase. Novosibirsk State Pedagogical University Bulletin, 2013, vol. 3, no. 5, pp. 136–143.
References: 

1. Anisimov V. N. Molecular and physiological mechanisms of aging. St. Petersburg: Science Publ., 2003. p. 468. (In Russian)
2. Ermakov S. P., Gavrilov N. S. Primary statistical analysis of data on survival of organisms. Results of Science and Technology. VINITI. Common problems of biology, 1987, vol. 6, pp. 230–276. (In Russian)
3. Krut'ko V. N., Slavin M. B., Smirnova T. M. Mathematical Foundations of Gerontology. Moscow: Editorial URSS Publ., 2002. pp 384. (In Russian)
4. Moskalev A. A., Krementsova A. V., Malysheva O. A. Melatonin influence on Drosophila melanogaster life span at different light regimes. Ekol. Genet., 2008, no. 3, pp. 22–30. (In Russian)
5. Moskalev A. A., Malysheva O. A. The role of transcription factors DFOXO, DSIR2 and HSP70 in lifespan alteration of Drosophila melanogaster in different light conditions. Ekol. Genet., 2010, no. 3, pp. 67–80. (In Russian)
6. Skulachev V. P. Evolution, mitochondria and oxygen. Soros Educational Journal, 1999, no. 9, pp. 4–10. (In Russian)
7. Helfand S. L., Rogina В.Genetics of aging in the fruit fly Drosophila melanogaster. Annu. Rev. Genet., 2003, vol. 37, pp. 329–348.
8. Honda Y, Tanaka M, Honda S. Redox regulation, gene expression and longevity. Geriatr Gerontol Int., 2010, vol. 10, pp. 59-69.
9. Le Bourg E. Oxidative stress, aging and longevity in Drosophila melanogaster. FEBS Letters, 2001, vol. 498, pp. 183–186.
10. Massie H. R, Whitney S.J. Preliminary evidence for photochemical ageing in Drosophila. Mech. Ageing Dev., 1991, vol. 58, pp. 37–48.
11. Orr W. C., Sohal R. S. Does overexpression of Cu, Zn-SOD extend life span in Drosophila melanogaster? Experimental gerontology, 2003, vol. 38, pp. 227–230.
12. Sheeba V., Sharma V. K., Shubha K., Chandrashekaran M. K., Joshi A. The effect of different light regimes on adult life span in Drosophila melanogaster is partly mediated through reproductive output. J. Biol. Rhythms., 2000, no. 5, pp. 380–392.
13. Sheeba V., Chandrashekaran M. K., Joshi A., Sharma V. K. Developmental plasticity of the locomotor activity rhythm of Drosophila melanogaster. J. Insect Physiol., 2002, vol. 48, pp. 25–32.
14. Staveley B. E., Phillips J. P., Hilliker A. J. Phenotypic consequences of copper-zinc superoxide dismutase overexpression in Drosophila melanogaster. Genome, 1990, vol. 33, pp. 867–872.
15. Wang C., Li Q., Redden D.T, Weindruchc R., Allisona D.B. Statistical methods for testing effects on «maximum lifespan». Mech. Ageing Dev., 2004, vol. 125, no. 9, pp. 629–632.

Date of the publication 26.10.2013