Novosibirsk State Pedagogical University Bulletin, 2013, No.5, pp.144-148
УДК 615.322

Study the influence of extraction conditions on elicitation of biologically active substances with antioxidant properties

Shubenkova E. 1 (Omsk, Russian Federation), Chzhu O. 1 (Omsk, Russian Federation), Lobova Y. 1 (Omsk, Russian Federation), Lutayeva I. 1 (Omsk, Russian Federation)
1 Omsk State Technical University

The paper presents the results of a study of antioxidant properties of plant extracts. The aim of the work was to study the effect of extraction conditions on the extraction of biologically active substances (BAS). The objects of investigation were water-alcohol and oil extracts of medicinal plants. Removing a complex of biologically active substances was performed by repeated two-phase extraction and ultrasonic extraction. The relative antioxidant activity of the extract was indicated as volume of extracts in milliliters, consumed for the titration of 1 ml of 0.05 N solution of potassium permanganate. Finally, conclusions are drawn on the impact of the extraction conditions on the content of biologically active substances with antioxidant properties in the resulting extracts.


biologically active substances, two-phase extraction, ultrasound, antioxidants, antioxidant properties.


1. Berdimuratov G.D. Biologically active substances of plants: isolation, separation, analysis / G.D. Berdimuratov [et al.] - Almaty: Kazakh National University Publishing House, 2006. – 438 p.
2. Maximova T.V. Method of antioxidant activity determination/ T.V. Maximova, I.N. Nikulin, V.P. Pakhomov, E.I. Shkarina etc - Patent RU 2170930. - Moscow Medical Academy. I.M. Sechenov. – Moscow, 2000.
3. Shubenkova E.G., Chzhu O.P. "Biochemical developments of extractions of biologically active substances". Applied and Fundamental Studies: Proceedings of the 2nd International Academic Conference. March 8-10, 2013, St. Louis, USA. Publishing House "Science & Innovation Center", 2013. 55-59.

Date of the publication 26.10.2013